Human Gene BAG6 (uc011gzj.2) Description and Page Index
  Description: Homo sapiens BCL2-associated athanogene 6 (BAG6), transcript variant 1, mRNA.
RefSeq Summary (NM_004639): This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Transcript (Including UTRs)
   Position: chr6_mann_hap4:2,949,688-2,963,366 Size: 13,679 Total Exon Count: 25 Strand: -
Coding Region
   Position: chr6_mann_hap4:2,949,791-2,962,429 Size: 12,639 Coding Exon Count: 24 

Page IndexSequence and LinksUniProtKB CommentsCTDMicroarrayRNA Structure
Protein StructureOther SpeciesmRNA DescriptionsOther NamesModel InformationMethods
Data last updated: 2013-06-14

+  Sequence and Links to Tools and Databases
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-  Comments and Description Text from UniProtKB
  ID: BAG6_HUMAN
DESCRIPTION: RecName: Full=Large proline-rich protein BAG6; AltName: Full=BAG family molecular chaperone regulator 6; AltName: Full=BCL2-associated athanogene 6; Short=BAG-6; Short=BAG6; AltName: Full=HLA-B-associated transcript 3; AltName: Full=Protein G3; AltName: Full=Protein Scythe;
FUNCTION: Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane.
FUNCTION: Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2).
FUNCTION: Can be released from tumor and dendritic cells in membrane vesicles or exosomes, and engage NCR3 thereby promoting natural killer cell (NK) activation and cytotoxicity.
SUBUNIT: Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET3/TRC35. Interacts with AIFM1, CTCFL, HSPA2 and p300/EP300. Interacts with ricin A chain. Interacts with L.pneumophila proteins Lpg2160 and LegU1. Interacts with NCR3.
INTERACTION: Q7Z434:MAVS; NbExp=2; IntAct=EBI-347552, EBI-995373;
SUBCELLULAR LOCATION: Cytoplasm, cytosol. Nucleus. Note=The C- terminal fragment generated by caspase-3 is cytoplasmic. Also found in extracellular vesicular exosomes in some tumor cells.
PTM: Cleavage by caspase-3 releases a C-terminal peptide that plays a role in ricin-induced apoptosis.
PTM: In case of infection by L.pneumophila, ubiquitinated by the SCF(LegU1) complex.
SIMILARITY: Contains 1 ubiquitin-like domain.
SEQUENCE CAUTION: Sequence=AAD18085.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=BAB63390.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=CAI18318.2; Type=Erroneous gene model prediction;

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  Microarray Expression Data
 
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GNF Expression Atlas 2 Data from U133A and GNF1H Chips

      
      
      
     
    
     
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GNF Expression Atlas 1 Human Data on Affy U95 Chips

      
    
     
      
    
    
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -140.20314-0.446 Picture PostScript Text
3' UTR -19.40103-0.188 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR021925 - DUF3538
IPR019954 - Ubiquitin_CS
IPR000626 - Ubiquitin_dom

Pfam Domains:
PF00240 - Ubiquitin family

SCOP Domains:
54236 - Ubiquitin-like

Protein Data Bank (PDB) 3-D Structure

1WX9
- NMR Chimera

4DWF
- X-ray Chimera

4EEW
- X-ray Chimera
Chimera help

ModBase Predicted Comparative 3D Structure on P46379
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
Jackson LabRGD    
Protein Sequence     
Alignment     

-  Descriptions from all associated GenBank mRNAs
  AK302695 - Homo sapiens cDNA FLJ60983 complete cds, highly similar to Large proline-rich protein BAT3.
BX647244 - Homo sapiens mRNA; cDNA DKFZp686L0653 (from clone DKFZp686L0653).
BC003133 - Homo sapiens HLA-B associated transcript 3, mRNA (cDNA clone MGC:3619 IMAGE:2957896), complete cds.
M33519 - Human HLA-B-associated transcript 3 (BAT3) mRNA, complete cds.
AK304879 - Homo sapiens cDNA FLJ61584 complete cds, highly similar to Large proline-rich protein BAT3.
AB384971 - Synthetic construct DNA, clone: pF1KB4636, Homo sapiens BAT3 gene for large proline-rich protein BAT3, complete cds, without stop codon, in Flexi system.
CU678390 - Synthetic construct Homo sapiens gateway clone IMAGE:100020517 5' read BAT3 mRNA.
DQ591812 - Homo sapiens piRNA piR-58924, complete sequence.

-  Other Names for This Gene
  Alternate Gene Symbols: A2ADJ7, A3KQ42, A3KQ44, A6NGY6, A6PWF7, B0UX84, BAG6_HUMAN, BAT3, G3, NM_004639, NP_004630, O95874, P46379, Q5HYL9, Q5SQ35, Q5SQ36, Q5SQ37, Q5SQ41, Q5SRP8, Q5SRP9, Q5STC1, Q5STX1, Q5STX3, Q96SA6, Q9BCN4
UCSC ID: uc011gzj.2
RefSeq Accession: NM_004639
Protein: P46379 (aka BAG6_HUMAN